منابع مشابه
Isolation of the lac repressor.
The realization that the synthesis of proteins is often under the control of repressorsl' 2 has posed a central question in molecular biology: What is the nature of the controlling substances? The scheme of negative control proposed by Jacob and Mionod envisages that certain genes, regulatory genes, make products that can act through the cytoplasm to prevent-the functioning of other genes. Thes...
متن کاملModeling the Lac repressor-operator assembly: the influence of DNA looping on Lac repressor conformation.
Repression of transcription of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by the simultaneous binding of LacR to two operators and the formation of a DNA loop. A recently developed theory of sequence-dependent DNA elasticity enables one to relate the fine structure of the LacR-DNA complex to a wide range of heretofore-unconnected experimental observations. Here, ...
متن کاملlac Operator nucleosomes. 2. lac Nucleosomes can change conformation to strengthen binding by lac repressor.
We have shown previously that lac repressor binds specifically and quantitatively to lac operator restriction fragments which have been complexed with histones to form artificial nucleosomes (203 base pair restriction fragment) or core particles (144 base pair restriction fragment. We describe here a quantitative method for determining the equilibrium binding affinities of repressor for these l...
متن کامل"Cold-sensitive" mutants of the Lac repressor.
Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.
متن کاملMutants that make more lac repressor.
The gene that makes the lac repressor functions at an extremely low rate. It synthesizes only a few thousandths of a per cent of the total protein of an E. coli cell: about 5-10 molecules each generation.1 In order to study this molecule more easily, we have sought to enhance the amount present in a cell. Two approaches have been successful. The repressor gene, the i gene, itself can be mutated...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1980
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(80)84978-2